Fig. 3
From: A novel candidate gene CLN8 regulates fat deposition in avian
Active region screening of the CLN8 promoter. A Different lengths fragment of CLN8 promoter cloned by PCR. B Plasmid digested of pGL3.1-Basic Vector by NheI and XhoI. C Relative luciferase activity of different promoter fragments. F1-F7: Fluorescence activity values detected after transfection of ICP1 cells with fluorescence vectors containing promoter truncated fragments of different lengths, using the pRL-TK as reference. Data are shown as mean ± SD of three biological replicates. An independent sample t-test was used to analyze the statistical differences between groups. a–cDifferent lowercase letters showed significant differences (P < 0.05) in relative luciferase activity between groups