Fig. 8

The synopsis of the model for future research on the pangenomes of livestock and poultry. In the future, livestock pangenome research should effectively combine TGS and NGS data. First, representative samples should be selected to extract high-quality DNA for TGS or NGS sequencing. Second, the high-quality genomes or haplotype-resolved assemblies are generated to construct the graph pangenome, then performs SV detection and identification. Thirdly, newly generated or existing NGS data in the database are compared with the pangenome for genotyping. Combining with multi-omics data, the SV affecting the important economic traits of livestock and poultry will be mined and verified from multiple angles and levels. Finally, the molecular markers found should first be validated at the cellular level. If possible, gene editing technology can be used to verify the effect of the functional gene on phenotype at the individual level