Fig. 6

SFN suppressed the expression of inflammatory factors Nrf2 and LC3 in mouse mammary glands following LPS treatment. BALB/c mice were administered vehicle (saline) or SFN (50 mg/kg, i.p.) on days 4 to 10 of lactation. On day 9 of lactation, mice were infused with 100 μL LPS (0.2 mg/mL) into the fourth mammary glands through the mammary ducts. After 24 h, mammary glands were collected from 10 mice for each group. A Representative images of mammary gland stained with H&E in mice (Bar = 50 μm). B–D qRT-PCR analysis of mammary glands specimen from 10 mice for each group to determine expression of inflammatory factors (n = 10). E Immunohistochemistry staining of Nrf2 is shown (Bar = 50 μm). F Immunofluorescence staining of LC3 puncta (Bar = 50 μm). One-way ANOVA, Dunnett's post-hoc test. Data are presented as the mean ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 and ^****P < 0.0001