Fig. 5
From: Dynamic chromatin architectures provide insights into the genetics of cattle myogenesis
The regulatory effect of RE on primary bovine myoblast (PBM) proliferation. A Visualization of integrative information about RE (highlighted in gray bar). B Validation of RE enhancer activity using a Dual-Luciferase Reporter System in PBMs. Data shown as means ± SD (n = 5). C PBMs isolated from Longissimus dorsi of fetal cattle. D–F Validation of the effects of RE on PBM proliferation using a CRISPR-mediated interference (CRISPRi) system. The expressions of HMGA2 and proliferation markers were significantly decreased (n = 9) (D). The number of EdU positive cells was clearly reduced (E). The percentage of PBMs in S phase was considerably decreased (n = 3) (F). CFM, fetal cattle muscle; CAM, adult cattle muscle; NCR, negative control region. *P < 0.05