Effects of high-grain diet feeding on fatty acid profiles in milk, blood, muscle, and adipose tissue, and transcriptional expression of lipid-related genes in muscle and adipose tissue of dairy cows

Table 8 Differentially expressed genes in abdominal adipose tissue due to high-grain diet feeding¹

Pathway	Gene symbol²	FDR	Log₂FC	Gene-ID
Fatty acid synthesis	FASN	< 0.001	1.375	24,950
	ACACA	< 0.001	1.011	23,708
	ACSBG2	0.037	1.683	9568
Linoleic acid metabolism	PLA2G2D1	< 0.001	2.239	2731
	CYP3A24	< 0.001	4.498	30,249
	CYP2E1	< 0.001	13.169	30,981
	HRAS-3	< 0.001	14.152	32,537
PPAR signalling pathway	UCP1	< 0.001	3.011	21,041
	ACSBG2	0.037	1.683	9568
	APO-A1	< 0.001	−1.230	18,573
	OLR1	0.009	−1.145	7294
	APO-C3	0.002	−1.003	18,572
	SLC27A2	0.047	−11.379	13,784
	ANGPTL4	< 0.001	−1.228	9509

¹Results based on RNA sequencing, n = 6. The gene expressions were considered to be significantly altered when fold change ≥ 2 and FDR < 0.05
²FASN Fatty acid synthetase, ACACA Acetyl-CoA carboxylase, ACSBG2 Long-chain-fatty-acid–CoA ligase ACSBG2 isoform X1, CYP3A24 Cytochrome P450 3A24, CYP2E1 Cytochrome P450 2E1, HRAS-3 HRAS-like suppressor 3, PLA2G2D1 Calcium-dependent phospholipase A2 PLA2G2D1 precursor, UCP1 Mitochondrial brown fat uncoupling protein 1, APO-A1 Apolipoprotein A-I preproprotein, OLR1 Oxidized low-density lipoprotein receptor 1, APO-C3 Apolipoprotein C-III precursor, SLC27A2 Very long-chain acyl-CoA synthetase, ANGPTL4 Angiopoietin-related protein 4 precursor

ISSN: 2049-1891