Growth performance, nutrient digestibility, intestinal morphology, cecal mucosal cytokines and serum antioxidant responses of broiler chickens to dietary enzymatically treated yeast and coccidia challenge

Table 5 Main effects of ETY and coccidia challenge on the relative expression of genes in the cecal mucosa, serum antioxidant markers and jejunal mucin yield in broiler chickens, d 21 post hatching

Item	Concentrations of ETY, g/kg			CS		SD^a	P-value
	Concentrations of ETY, g/kg			CS			Challenge	ETY		Challenge × ETY interaction
	0	1	2	No-challenge	Challenge		Challenge	L	Q	Challenge × ETY interaction
Relative gene expression
TNFα	0.91	0.93	0.85	0.75	1.04	0.188	< 0.001	0.385	0.361	0.297
CLDN1	0.85	0.89	0.88	0.85	0.90	0.496	0.739	0.919	0.824	0.251
IL-1β	0.90	0.77	0.68	0.61	0.95	0.364	0.002	0.084	0.855	0.979
OCLN	0.68	1.10	1.15	0.97	0.98	0.797	0.919	0.045	0.333	0.535
IL-10	0.59	0.82	0.70	0.32	1.09	0.730	0.001	0.705	0.410	0.491
IL-6	0.91	0.58	0.55	0.33	1.03	0.877	0.005	0.220	0.546	0.733
No. of replicates	16	16	15	24	23
Serum antioxidants
Catalase, U/mL	2.88	9.95	7.88	8.85	4.96	6.744	0.049	0.044	0.029	0.265
SOD, U/mL	629.27	574.94	529.44	661.03	494.73	366.514	0.075	0.314	0.977	0.095
GPX, U/mL	12.12	12.35	12.15	12.22	12.19	0.199	0.505	0.589	0.002	0.920
Mucin, mg/g jejunal digesta	383.63	348.96	362.93	309.36	420.99	77.002	< 0.001	0.438	0.295	0.627
No. of replicates	13	13	14	20	20

ETY Enzymatically treated yeast, CS Challenge state, L Linear effect of ETY, Q Quadratic effect of ETY, TNFα Tumor Necrosis factor α, CLDN1 Claudin 1, IL Interleukin, OCLN Occludin, SOD Superoxide dismutase, GPX Glutathione peroxidase
^aSD Standard deviation

ISSN: 2049-1891