Growth performance, nutrient digestibility, intestinal morphology, cecal mucosal cytokines and serum antioxidant responses of broiler chickens to dietary enzymatically treated yeast and coccidia challenge

Table 2 Sequences of primers used for the real-time PCR analysis

Target gene	Primer sequence (5′→3′)	Annealing temperature, °C	GenBank accession number	References
IL-1β	F: GCATCAAGGGCTACAAGCTC	57.7	NM_204524	Adedokun et al. [17]
IL-1β	R: CAGGCGGTAGAAGATGAAGC
IL-6	F: GAACGTCGAGTCTCTGTGCTAC	61.8	NM_204628	Current study
IL-6	R: CACCATCTGCCGGATCGT
IL-10	F: GGACTATTTTCAATCCAGGGACG	53.4	NM_001004414.2	Daneshmand et al. [18]
IL-10	R: GGGCAGGACCTCATCTGTGTAG
CLDN1	F: CAGACTCTAGGTTTTGCCTT	52.0	NM_001013611.2	Goo et al. [19]
CLDN1	R: AATCTTTCCAGTGGCGATAC
OCLN	F: TCGTGCTGTGCATCGCCATC	53.4	NM_205128.1	Goo et al. [19]
OCLN	R: CGCTGGTTCACCCCTCCGTA
TNFα	F: AGATGGGAAGGGAATGAACC	55.7	AY765397	Adedokun et al. [17]
TNFα	R: ACTGGGCGGTCATAGAACAG
GAPDH	F: ATGACCACTGTCCATGCCATCA	59.0	NM_204305.1	Adedokun et al. [17]
GAPDH	R: AGGGATGACTTTCCCTACAGCCTT

F Forward primer, R Reverse primer, IL Interleukin, CLDN1 Claudin 1, OCLN Occludin, TNFα Tumor necrosis factor alpha, GAPDH Glyceraldehyde-3-phosphate dehydrogenase

ISSN: 2049-1891