Fig. 8

MFAEs attenuated hepatocyte injury and ferroptosis via the Nrf2-dependent pathway. A ALT, AST, LDH, IL-6, TNF-α and IL-1β levels in cell supernatants. B Representative image of immunofluorescence staining for ROS, Fe²⁺ and LPO in HepG2 cells. Bar, 100 μm (100×), 25 μm (640×). n = 3 per group. C Western blot analysis of Nrf2, SLC7A11, GPX4 and ACSL4. β-actin served as loading control. Results are expressed as mean ± SD. Independent sample t test was used for statistical analysis. ^* or ^**indicates a significant difference between the two groups, ns indicates a nonsignificant difference between the two groups. ^*P < 0.05, ^**P < 0.01