Fig. 7
From: Mori fructus aqueous extracts attenuates liver injury by inhibiting ferroptosis via the Nrf2 pathway
MFAEs downregulated Fe2+ accumulation and inflammation in vitro. A Outline of experimental procedures. B ALT, AST, LDH, IL-6, TNF-α and IL-1β levels in cell supernatants. C FITC/PI double staining results were detected by flow cytometry. n = 3 per group. D Representative image of immunofluorescence staining for ROS, Fe2+ and LPO in HepG2 cells. Bar, 100 μm (100×), 25 μm (640×). E Western blot analysis of Nrf2, SLC7A11, GPX4 and ACSL4. β-actin served as loading control. *Indicates a significant difference compared with the control group (*P < 0.05, **P < 0.01). #Indicates a significant difference compared with the 10% CCl4 group (#P < 0.05, ##P < 0.01)