Fig. 5
From: Mori fructus aqueous extracts attenuates liver injury by inhibiting ferroptosis via the Nrf2 pathway
MFAEs suppresses fibrosis and inflammation in CCl4-induced chronic liver injury mice. A Masson staining and immunohistochemistry staining for α-SMA images of liver sections and fibrosis scores in the indicated mice. Bar, 100 μm. Black arrow: pseudolobule, yellow arrow: fibrosis, red triangle: positive area. B Serum HA and liver HYP contents in indicated mice. C Western blot analysis of MMP1, MMP9, TIMP1, VEGFA and α-SMA in the livers of indicated mice. β-actin served as loading control. D Semiquantitative analysis. E Serum IL-6, TNF-α and IL-1β in indicated mice. F Western blot analysis of total and nuclear HMGB1 and NF-κBp65 in the livers of indicated mice. Lamin B1 and β-actin served as loading controls. G TUNEL analysis in the livers of indicated mice. Bar, 100 μm (100×), 50 μm (400×). *Indicates a significant difference compared with the control group (*P < 0.05, **P < 0.01). #Indicates a significant difference compared with the 10% CCl4 group (#P < 0.05, ##P < 0.01)