Fig. 3
From: Mori fructus aqueous extracts attenuates liver injury by inhibiting ferroptosis via the Nrf2 pathway
MFAEs boost antioxidant capacity in CCl4-induced acute liver injury mice. A Serum and liver antioxidants levels of indicated mice. B Western blot analysis of total and nuclear Nrf2, Keap-1, HO-1, NQO-1, iNOS, Ferritin, ACSL4 and GPX4 in the livers of indicated mice. Lamin B1 and β-actin served as loading control. C Representative image of immunohistochemical staining for Nrf2, HO-1, NQO-1 and Keap-1 in the livers of indicated mice. Bar, 50 μm. Black arrow: congestion, yellow arrow: hepatocyte edema, red triangle: positive area. D Relative mRNA levels of Nfe2l2, Hmox-1, Nqo-1 and Keap-1. *Indicates a significant difference compared with the control group (*P < 0.05, **P < 0.01). #Indicates a significant difference compared with the 0.3% CCl4 group (#P < 0.05, ##P < 0.01)