Fig. 2
From: Mori fructus aqueous extracts attenuates liver injury by inhibiting ferroptosis via the Nrf2 pathway
MFAEs attenuated fibrosis and inflammation in CCl4-induced acute liver injury mice. A Masson staining images of liver sections and fibrosis scores in indicated mice. Bar, 100 μm. Black arrow: congestion, yellow arrow: hepatocyte edema, red triangle: positive area. B Liver HYP, LDH and γ-GT levels in indicated mice. C Serum LDH and γ-GT levels in indicated mice. D Western blot analysis of MMP1, MMP9, TIMP1 and VEGFA in the livers of indicated mice. β-actin served as loading control. n = 3 per group. E Serum IL-6, TNF-α and IL-1β content in indicated mice. n = 6 per group. F Relative mRNA levels of Tnf and Il1b. G Western blot analysis of total and nuclear NF-κBp65 in the livers of indicated mice. Lamin B1 and β-actin served as loading controls. n = 3 per group. H TUNEL analysis in the livers of indicated mice. Bar, 100 μm (100×), 50 μm (400×). *Indicates a significant difference compared with the control group (*P < 0.05, **P < 0.01). #Indicates a significant difference compared with the 0.3% CCl4 group (#P < 0.05, ##P < 0.01)