Mannan oligosaccharides alleviate oxidative injury in the head kidney and spleen in grass carp (Ctenopharyngodon idella) via the Nrf2 signaling pathway after Aeromonas hydrophila infection

Table 1 Composition and nutrient content of the diet (dry matter basis), %

Ingredients	Content, %	Nutrient	Content, %
Fish meal	7.80	Crude protein^d	28.69
Gelatin	6.00	Crude lipid^d	5.36
Soybean protein concentrated	26.00	n-3^d	1.04
Corn starch	19.90	n-6^d	0.96
α-starch	24.00	Available phosphorus^d	0.40
Fish oil	2.34
Soybean oil	1.81
Cellulose	5.00
Ca(H₂PO4)₂	1.30
Vitamin premix^a	1.00
Mineral premix^b	2.00
MOS premix^c	1.00
Choline chloride (50%)	1.00
Ethoxyquin (30%)	0.05
DL-Met (99%)	0.61
L-Trp (99%)	0.08
Thr (98.5%)	0.11

^aPer kilogram of vitamin premix (g/kg): retinyl acetate (500,000 IU/g), 0.39; cholecalciferol (500,000 IU/g), 0.40; DL-α-tocopherol acetate (50%), 23.23; menadione (22.9%), 0.83; cyanocobalamin (1%), 0.94; D-biotin (2%), 0.75; folic acid (95%), 0.42; thiamine nitrate (98%), 0.09; ascorhyl acetate (95%), 9.77; niacin (99%), 4.04; meso-inositol (98%), 19.39; calcium-D-pantothenate (98%), 3.85; riboflavin (80%), 0.73; pyridoxine hydrochloride (98%), 0.62. All ingredients were diluted with corn starch to 1 kg
^bPer kilogram of mineral premix (g/kg): MnSO₄⋅H₂O (31.8% Mn), 2.6590; MgSO₄⋅H₂O (15.0% Mg), 200.0000; FeSO₄⋅H₂O (30.0% Fe), 12.2500; ZnSO₄⋅H₂O (34.5% Zn), 8.2460; CuSO₄⋅5H₂O (25.0% Cu), 0.9560; KI (76.9% I), 0.0650; Na₂SeO₃ (44.7% Se), 0.0168. All ingredients were diluted with corn starch to 1 kg
^cMOS premix (mg/kg): premix was added to obtain graded levels of MOS
⁴Crude protein and crude lipid content were measured value. Available phosphorus, n-3 and n-6 contents calculated according to NRC (2011) [31]

ISSN: 2049-1891