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Table 4 Effects of supplementation of DHA-rich microalgal oil, EPA-rich microalgal biomass, and calcidiol on plasma and tissue lipid profiles of broiler chickens at week 6 in Exp. 2

From: Supranutrition of microalgal docosahexaenoic acid and calcidiol improved growth performance, tissue lipid profiles, and tibia characteristics of broiler chickens

Item 

BD

BD + DHA

BD + DHA + EPA

BD + DHA + Cal

BD + DHA + EPA + Cal

SEM

P-value

Plasma

 PL, mg/dL

78.9

61.7

67.1

76.3

70.7

4.50

0.08

 TG, mg/dL

40.4b

27.8ab

18.7a

39.2b

28.8ab

3.76

 < 0.01

 TC, mg/dL

102b

89.6ab

83.8a

90.3ab

79.8a

3.53

 < 0.01

 NEFA, µmol/mL

87.3b

62.0a

76.6ab

85.6b

78.8ab

4.21

 < 0.01

Liver

 PL, mg/g tissue

14.6

12.3

12.5

14.7

13.7

1.74

0.22

 TG, mg/g protein

71.4b

68.2b

58.7a

70.9b

66.1ab

6.12

 < 0.01

 TC, mg/g protein

14.7

13.3

13.6

14.1

13.6

1.37

0.95

 NEFA, µmol/g protein

46.0

41.5

34.1

42.1

38.8

3.10

0.15

Breast

 PL, mg/g tissue

3.60

2.89

3.09

3.38

2.76

0.19

0.32

 TG, mg/g protein

23.8b

22.9b

18.1a

23.1b

22.9b

2.11

0.04

 TC, mg/g protein

5.82

4.61

4.69

5.41

5.59

0.30

0.05

 NEFA, µmol/g protein

5.08b

4.67ab

3.78a

4.50ab

4.44ab

0.91

0.03

Thigh

 PL, mg/g tissue

3.34

2.47

2.92

2.83

2.56

0.32

0.12

 TG, mg/g protein

26.6b

24.5ab

21.5a

24.2ab

24.0ab

2.16

0.01

 TC, mg/g protein

4.00

3.93

3.63

3.88

3.78

0.11

0.21

 NEFA, µmol/g protein

5.18

4.99

4.58

5.07

4.53

1.01

0.90

  1. 0–3 weeks: BD = Corn-soybean basal diet; BD + DHA = BD + 1.5 g of DHA oil/kg; BD + DHA + EPA = BD + DHA + 0.3 g/kg Nannochloropsis sp. CO18; BD + DHA + Cal = BD + DHA + 6000 IU calcidiol/kg; BD + DHA + EPA + Cal = BD + DHA + EPA (0.3 g/kg Nannochloropsis sp. CO18) + 6000 IU calcidiol/kg
  2. 4–6 weeks: BD = Corn-soybean basal diet; BD + DHA = BD + 3.0 g DHA oil/kg; BD + DHA + EPA = BD + DHA + 0.6 g/kg Nannochloropsis sp. CO18; BD + DHA + Cal = BD + DHA + 12,000 IU calcidiol/kg; BD + DHA + EPA + Cal = BD + DHA + EPA (0.6 g/kg Nannochloropsis sp. CO18) + 12,000 IU calcidiol/kg
  3. a,bMeans bearing the different superscripts in a row differ (P < 0.05)
  4. Values are expressed as means of 6 birds/treatment and data were analyzed using one-way ANOVA