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Table 1 Effects of supplementation of calcidiol, DHA-rich microalgal biomass or oil, and EPA-rich microalgal biomass on growth performance of broiler chickens in Exp. 1 and 2 (0–6 weeks)

From: Supranutrition of microalgal docosahexaenoic acid and calcidiol improved growth performance, tissue lipid profiles, and tibia characteristics of broiler chickens

Exp. 1

Parameters

BD

BD + Cal

BD + DHA

BD + Cal + DHA

SEM

P-value

 

BW 0 d, g/chick

41.9

41.8

41.8

41.7

0.170

0.85

 

BW 0–6 weeks, g/chick

2910a

3230b

3090ab

3160ab

51.20

< 0.01

 

BWG 0–6 weeks, g/chick

2870a

3190b

3040ab

3120ab

57.25

< 0.01

 

FI 0–6 weeks, g/chick

4860a

5050ab

5240ab

5450b

115.4

< 0.01

 

Gain:Feed

0.590a

0.630b

0.580a

0.570a

0.008

< 0.01

 

Exp. 2

 

BD

BD + DHA

BD + DHA + EPA

BD + DHA+ Cal

BD + DHA + EPA + Cal

SEM

P-value

BW 0 d, g/chick

42.3

42.3

42.4

42.4

42.4

0.07

0.64

BW 0–6 weeks, g/chick

2520a

2600ab

2770b

2530ab

2590ab

62.82

0.04

BWG 0–6 weeks, g/chick

2480a

2560ab

2730b

2490ab

2550ab

62.84

0.04

FI 0–6 weeks, g/chick

3880a

3870a

4410b

4080ab

4470b

121.8

< 0.01

Gain:Feed

0.640ab

0.660b

0.620ab

0.610ab

0.570a

0.02

0.02

  1. BW Body weight, BWG Body weight gain, FI: Feed intake
  2. Exp. 1; BD = Corn-soybean basal diet; BD + Cal = BD + 10,000 IU calcidiol/kg of diet; BD + DHA = BD + 1% DHA-rich microalgal biomass; BD + Cal + DHA = BD + Cal + 1% DHA-rich microalgal biomass
  3. Exp. 2; 0–3 weeks: BD = Corn-soybean basal diet; BD + DHA = BD + 1.5 g of DHA oil/kg; BD + DHA + EPA = BD + DHA + 0.3 g/kg Nannochloropsis sp. CO18; BD + DHA + Cal = BD + DHA + 6000 IU calcidiol/kg; BD + DHA + EPA + Cal = BD + DHA + EPA (0.3 g/kg Nannochloropsis sp. CO18) + 6000 IU calcidiol/kg
  4. Exp. 2; 4–6 weeks: BD = Corn-soybean basal diet; BD + DHA = BD + 3.0 g DHA oil/kg; BD + DHA + EPA = BD + DHA + 0.6 g/kg Nannochloropsis sp. CO18; BD + DHA + Cal = BD + DHA + 12,000 IU calcidiol/kg; BD + DHA + EPA + Cal = BD + DHA + EPA (0.6 g/kg Nannochloropsis sp. CO18) + 12,000 IU calcidiol/kg
  5. a,bMeans bearing the different superscripts in a row differ (P < 0.05)
  6. Data are expressed as means (n = 6 cages and 6 birds/cage) and were analyzed by two-way and one-way ANOVA in Exp. 1 and 2, respectively