Table 1 Primer sequences used for real-time PCR
Gene | Primer | Nucleotide sequences (5′ to 3′) |
|---|---|---|
β-actin | F | GCGTAGCATTTGCTGCATGA |
R | GCGTGTGTGTAACTAGGGGT | |
ZO-1 | F | CTCCAGGCCCTTACCTTTCG |
R | GGGGTAGGGGTCCTTCCTAT | |
Occludin | F | CAGGTGCACCCTCCAGATTG |
R | TATGTCGTTGCTGGGTGCAT | |
Claudin-1 | F | TCGACTCCTTGCTGAATCTG |
R | TTACCATACCTTGCTGTGGC | |
IL-1β | F | GCCAGTCTTCATTGTTCAGGTTT |
R | CCAAGGTCCAGGTTTTGGGT | |
IL-6 | F | TCCAATCTGGGTTCAATCA |
R | TCTTTCCCTTTTGCCTCA | |
IL-8 | F | TACGCATTCCACACCTTTC |
R | GGCAGACCTCTTTTCCATT | |
IL-10 | F | TCGGCCCAGTGAAGAGTTTC |
R | GGAGTTCACGTGCTCCTTGA | |
IL-17 | F | CTCTCGTGAAGGCGGGAATC |
R | GTAATCTGAGGGCCGTCTGG | |
TNF-α | F | CGTCGCCCACGTTGTAGCCAAT |
R | GCCCATCTGTCGGCACCACC | |
AhR | F | CATGCTTTGGTCTTTTATGC |
R | TTCCCTTTCTTTTTCTGTCC | |
CYP1A1 | F | CCTTCACCATCCCTCACAGT |
R | ATCACCTTTTCACCCAGTGC | |
CYP1B1 | F | AATAACGGGGGAAATTCCTG |
R | CACCGAAACACAATGCAATC | |
RegIIIγ | F | AACCTGGATGGGTGCAGACGTG |
R | TTGGTTCCAAGCCCTCGGTG | |
IL-22 | F | CTACATCACCAACCGCACCT |
R | TCAGAGTTGGGGAACAGCAC |