Yeast hydrolysate attenuates lipopolysaccharide-induced inflammatory responses and intestinal barrier damage in weaned piglets

Table 2 Primer sequences used for real-time PCR

Gene	Primer sequence (5’ →3’)	Product length, bp	Accession number
β-actin	F: TCTGGCACCACACCTTCT	114	DQ178122
β-actin	R: TGATCTGGGTCATCTTCTCAC	114	DQ178122
ZO-1	F: CAGCCCCCGTACATGGAGA	114	XM_005659811
ZO-1	R: GCGCAGACGGTGTTCATAGTT	114	XM_005659811
OCC	F: CTACTCGTCCAACGGGAAAG	158	NM_001163647.2
OCC	R: ACGCCTCCAAGTTACCACTG	158	NM_001163647.2
ClDN-1	F: TCTTAGTTGCCACAGCATGG	106	NM001244539
ClDN-1	R: CCAGTGAAGAGAGCCTGACC	106	NM001244539
MUC2	F: GGTCATGCTGGAGCTGGACAGT	181	XM_003122394.1
MUC2	R: TGCCTCCTCGGGGTCGTCAC	181	XM_003122394.1
IL-1β	F: CAGCTGCAAATCTCTCACCA	112	NM_214055.1
IL-1β	R: TCTTCATCGGCTTCTCCACT	112	NM_214055.1
TNF-α	F: CGTGAAGCTGAAAGACAACCAG	121	NM_214022.1
	R: GATGGTGTGAGTGAGGAAAACG
	R: CAGGCTTCCGTCATCTGGTT

CLDN-1 Claudin-1, OCC Occludin, MUC2 Mucin2, IL-1β interleukin-1β, TNF-α Tumor necrosis factor-α, ZO-1 Zonula occludens-1

ISSN: 2049-1891