Fig. 6

YTHDF1 regulate the translation of ADIPOQ in IMF cells. a The protein levels of ADIPOQ in porcine intramuscular preadipocytes transfected with siControl or siYTHDF1 (48 h). b The protein levels of ADIPOQ after overexpression YTHDF1 (48 h). c The protein levels of YTHDF1 of porcine intramuscular preadipocytes transfected with control or YTHDF1-FLAG plasmid (48 h). d RIP analysis of the interaction of ADIPOQ with FLAG in porcine intramuscular preadipocytes transfected with YTHDF1-FLAG plasmid. Enrichment of ADIPOQ with FLAG was measured by qPCR and normalized to input. e The protein levels of YTHDF1 of 293 T cells transfected with WT or MUT of ADIPOQ-3’UTR or YTHDF1 overexpression plasmid (48 h). f Relative luciferase activity of WT or MUT of ADIPOQ-3’UTR or YTHDF1 overexpression in 293 T cells, n = 3. g The mRNA expression levels of ADIPOQ of porcine intramuscular preadipocytes with WT or MUT of ADIPOQ-CDS or YTHDF1-overexpression plasmid (48 h), n = 3. h The protein expression levels of ADIPOQ of porcine intramuscular preadipocytes with WT or MUT of ADIPOQ-CDS or YTHDF1-overexpression plasmid (48 h). i and j TAG content and Oil Red O staining of porcine intramuscular preadipocytes with WT or MUT of ADIPOQ-CDS or YTHDF1 overexpression plasmid at 8 d after adipogenic induction, n = 3. k RT-qPCR of PPARγ, CEBPβ and aP2 of porcine intramuscular preadipocytes with WT or MUT of ADIPOQ-CDS or YTHDF1 overexpression plasmid at 8 d after adipogenic induction, n = 3. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001