Fig. 5

ADIPOQ promotes adipogenesis of preadipocytes in a m⁶A-dependent manner. a m⁶A-IP-qPCR analysis of ADIPOQ-3’UTR WT or MUT (A to T mutation) in 293 T cells, n = 3. b m⁶A-IP-qPCR analysis ADIPOQ-CDS WT or MUT (C to T mutation) in porcine intramuscular preadipocytes, n = 3. c Relative luciferase activity of WT or MUT of ADIPOQ-3’UTR in 293 T cells, n = 3. d The mRNA expression levels of ADIPOQ of porcine intramuscular preadipocytes with NC, WT or MUT of ADIPOQ-CDS, n = 3. e The protein expression levels of ADIPOQ of porcine intramuscular preadipocytes with NC, WT or MUT of ADIPOQ-CDS. f and g TAG content and Oil Red O staining of porcine intramuscular preadipocytes with NC, WT or MUT of ADIPOQ-CDS at 8 d after adipogenic induction, n = 3. h RT-qPCR of PPARγ, CEBPβ and aP2 of porcine intramuscular preadipocytes with NC, WT or MUT of ADIPOQ-CDS at 8 d after adipogenic induction, n = 3. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001