Fig. 4

ADIPOQ promote adipogenesis of preadipocytes in vitro. a Workflow of porcine intramuscular adipocytes inducing in vitro. b Protein levels of ADIPOQ in intramuscular preadipocytes at 0, 2, 4 and 8 d during adipogenesis. c The mRNA levels of ADIPOQ (48 h) after siRNA transfection of porcine intramuscular preadipocytes, n = 3. d The protein expression levels of ADIPOQ (48 h) after siRNA transfection of porcine intramuscular preadipocytes. e–g TAG content and Oil Red O staining of siADIPOQ at 8 d after adipogenic induction, n = 3. h RT-qPCR of PPARγ, CEBPβ and aP2 of siADIPOQ at 8 d after adipogenic induction, n = 3. i The mRNA expression levels of ADIPOQ after overexpression ADIPOQ (48 h), n = 3. j The protein expression levels of ADIPOQ after overexpression ADIPOQ (48 h). k, l TAG content and Oil Red O staining of ADIPOQ-overexpression at 8 d after adipogenic induction, n = 3. m RT-qPCR of PPARγ, CEBPβ and aP2 of ADIPOQ overexpression at 8 d after adipogenic induction, n = 3. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001