Fig. 3
RNA expression differentially and m6A modification differentially genes between High and Low. a Venn diagram of m6A modified genes across High and Low groups. b Volcano plot of m6A modified differential gene, P < 0.05 and fold change > 1.5 were marked as differentially methylated genes (blue and red), fold change value is calculated by High/Low. c GO and KEGG pathways of down (blue) and up (red) regulated m6A modification genes. d Venn diagram and (e) four quadrant diagram of methylated and RNA expression differential genes (P < 0.05 and fold change > 1.5) between High and Low group, 70 genes were observed significantly co-differential in e. f and g m6A methylation and mRNA expression of ADIPOQ gene between High and Low group, n = 8. Normalized read count was employed for comparing the level of m6A methylation between High and Low. Normalized read Count = SRN/ITR, SRN is site of reads number, while ITR is individual of total reads. SRN was counted using SAMtools v1.11 bedcov, ITR was inferred using SAMtools v1.11 flagstats based on BAM file. Input and IP data were both under the same pipeline of normalization. h Protein level of ADIPOQ between High and Low, n = 3