Adjusting phosphate feeding regimen according to daily rhythm increases eggshell quality via enhancing medullary bone remodeling in laying hens

Table 1 Composition and nutrient concentrations of basal diet (%, unless noted, as-is basis)

Item	Low phosphorus	Regular phosphorus
Ingredients
Corn	56.69	56.69
Soybean meal	25.77	25.77
Distillers dried grains with solubles	4.00	4.00
Calcium carbonate	9.73	9.04
Dicalcium phosphate	-	1.15
Soybean oil	1.51	1.51
Sodium chloride	0.26	0.26
DL-Methionine	0.18	0.18
Choline chloride	0.15	0.15
Montmorillonite	0.71	0.25
Premix¹	1	1
In total	100.00	100.00
Nutrient levels
Metabolizable energy, kcal/kg (calculated)	2,600	2,600
Crude protein (calculated)	16.5	16.5
Total phosphorus (calculated/analyzed)	0.34/0.34	0.53/0.49
Non-phytate phosphorus (calculated)	0.14	0.32
Calcium (calculated/analyzed)	3.50/3.47	3.50/3.52

¹Provided per kilogram of diet: manganese 60 mg, copper 8 mg, zinc 80 mg, iodine 0.35 mg, selenium 0.3 mg, vitamin A 8000 IU, vitamin E 30 mg, vitamin K₃ 1.5 mg, thiamine 4 mg, riboflavin 13 mg, pantothenic acid 15 mg, nicotinamide 20 mg, pyridoxine 6 mg, biotin 0.15 mg, folic acid 1.5 mg, and cobalamin 0.02 mg

ISSN: 2049-1891