Fig. 6

Daily dynamic phosphorus feeding regimen improved the rhythm of MB mineralization markers in 70-week-old Hy-Line Brown laying hens. A) ALPL mRNA (n = 6 per group); B) RUNX2 mRNA (n = 6 per group); C) BGLAP mRNA (n = 6 per group); D) SLC1A2 mRNA (n = 6 per group). Data are presented as the mean ± SEM. P < 0.05 by one-way ANOVA followed by Duncan's multiple range tests, the letters (A, B) and the letters (a, b) indicate significant differences among all treatment groups chosen from 6 h post-oviposition and 18 h post-oviposition, respectively (P < 0.05). The significance of difference between 6 h and 18 h post-oviposition for each group was analyzed using two-tailed students’ t-tests, ^*P < 0.05, ^**P < 0.01. RR, provided with regular phosphorus diet at both 09:00 and 17:00 for 12 weeks; RL, provided with regular phosphorus diet at 09:00 and low phosphorus diet at 17:00 for 12 weeks; LR, provided with low phosphorus diet at 09:00 and regular phosphorus diet at 17:00 for 12 weeks; LL, provided with low phosphorus diet at both 09:00 and 17:00 for 12 weeks. ALPL, alkaline phosphatase; BGLAP, gamma-carboxyglutamate protein; COL1A2, collagen type I alpha 2 chain; MB, medullary bone; RUNX2, runt related transcription factor 2