Fig. 4

Daily dynamic phosphorus feeding regimen increased uterine calcium transportation and eggshell quality in 70-week-old Hy-Line Brown laying hens for 12 weeks. A) Eggshell thickness (n = 62−66 per group); B) eggshell strength (n = 62−66 per group); C) egg specific gravity (n = 61−66 per group); D) shell index (n = 62−63 per group); D) western blot analysis and statistical analysis of protein abundances of ACTB, TRPV6 and CaBP-D28k in the uterus collected from 18 h post-oviposition (n = 3 per group), all samples were normalized to their respective ACTB levels of each sample. Data are presented as the mean ± SEM. P < 0.05 by one-way ANOVA followed by Duncan's multiple range tests and the letters (a−c) indicate significant differences among all treatment groups (P < 0.05). RR, provided with regular phosphorus diet at both 09:00 and 17:00 for 12 weeks; RL, provided with regular phosphorus diet at 09:00 and low phosphorus diet at 17:00 for 12 weeks; LR, provided with low phosphorus diet at 09:00 and regular phosphorus diet at 17:00 for 12 weeks; LL, provided with low phosphorus diet at both 09:00 and 17:00 for 12 weeks. ACTB, β-actin; CaBP-D28k, Calbindin D‐28k; TRPV6, Transient receptor potential vanilloid 6