Fig. 3

Diurnal rhythms of the intake and excretion of calcium and phosphorus in 40-week-old Hy-Line Brown laying hens. A) Calcium intake (n = 15 per group); B) phosphorus intake (n = 15 per group); C) total excretion of calcium (n = 15 per group); D) dry excrete concretion of calcium (n = 15 per group); E) total excretion of phosphorus (n = 15 per group); F) dry excrete concretion of phosphorus (n = 15 per group); G) representative western blots and statistical analysis of protein abundances of ACTB, PiT1, PiT2 and NPt2a in the kidney (n = 4 per group) , all samples were normalized to their respective ACTB levels of each sample. White and black bars represent the light and dark. Data are presented as the mean ± SEM. P < 0.05 by one-way ANOVA followed by Duncan's multiple range tests and the letters (a–d) indicate significant differences among all treatment groups (P < 0.05). ACTB, β-actin; NPt2a, Type 2a sodium-phosphate co-transporters; PiT1, Type III sodium-dependent phosphate transporter 1; PiT2, Type III sodium-dependent phosphate transporter 2