Fig. 1

Diurnal rhythms of serum calcium and phosphorus levels and uterine protein expressions in 40-week-old Hy-Line Brown laying hens during the egg laying cycle. A) Serum calcium (n = 9 per group); B) serum phosphorus (n = 9 per group); C) protein expression of TRPV6 and CaBP-D28k (n = 4 per group). White and black bars represent the light and dark. Data are presented as the mean ± SEM. P < 0.05 by one-way ANOVA followed by Duncan's multiple range tests and the letters (a–d) indicate significant differences among all treatment groups (P < 0.05). ACTB, β-actin; CaBP-D28k, Calbindin D‐28k; TRPV6, Transient receptor potential vanilloid 6