Fig. 4

Relative expression of Sirt3 mRNA and protein in different treatment groups. The control group (Control-PEX3) was transfected with PEX3 empty plasmid but not treated with BHBA; the Control-PEX3-Sirt3 group was transfected with PEX3-Sirt3 plasmid but not treated with BHBA; the BHBA-PEX3 group was transfected with PEX3 empty plasmid with BHBA treatment; the BHBA-PEX3-Sirt3 group was transfected with PEX3-Sirt3 plasmid with BHBA treatment. A Sirtuin 3 mRNA relative expression in the control and BHBA-2.4 mmol/L groups (n = 3). B Schematic protocol for Sirt3 overexpression with BHBA treatment. C The relative expression of Sirt3 mRNA in different groups following Sirt3 overexpression with BHBA treatment (n = 3). D–E The relative expression of Sirt3 protein in different groups (n = 3). For Fig. 4 A and E (left), we used Student t-tests for significant difference analysis (^*P < 0.05; ^**P < 0.01); for Fig. 4 C and E (right), we used one-way ANOVA for significant difference analysis (the different lowercases represent P < 0.05)