Fig. 5

Functional prediction of the rumen microbiota based on PICRUSt2 (a) and Tax4Fun (b) is performed on 16S rDNA data. Prediction of microbial functional differences based on Tax4Fun by the Welch's t-test in 16S rDNA sequencing (c). Comparisons of the abundance of CAZymes genes of rumen microbiomes in the F and G groups by the Welch's t-test in metagenomic sequencing (d). “F” means indoor feeding group; “G” means grazing group