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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: RNA-seq analysis reveals the critical role of the novel lncRNA BIANCR in intramuscular adipogenesis through the ERK1/2 signaling pathway

Fig. 5

Identification of BIANCR as a candidate lncRNA A GO (left) and KEGG (right) analysis of lncRNA MSTRG.16361.1. The top 20 enriched terms or pathways are showed according to P-values. B The potential regulatory network of lncRNA MSTRG.16361.1. Pink diamond, yellow rectangle, and cyan circle represents lncRNA, GO term/KEGG pathway, and Genes respectively. C Chromosome location and transcriptional structure of BIANCR. D The expression levels of BIANCR in various tissues of calves (left), adult cattle (mid) and comparison (right) between calves and adult cattle. E The expression levels of BIANCR in different adipogenesis stage. F qRT-PCR detection of BIANCR in the cytoplasmic and nuclear fractions of Intramuscular adipocytes. U6 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) provide as cytoplasmic and nuclear localization control respectively. G The knockdown of BIANCR utilizing RNA interference. Results are presented as the means ± SD, n = 3, *P < 0.05; ** P < 0.01; different lowercase letters indicate significant differences (P < 0.05)

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891