Fig. 9
Effects of PCA and Que on protein expression of necroptosis signals after infection with ETEC K88 in IPEC-1 cells. Cells were pre-treated with 40 μmol/L PCA or 10 μmol/L Que for 24 h and then infected with or without 1 × 108 ETEC K88/mL for 2 h. a–k Protein expression of t-RIP1, t-RIP3, t-MLKL, p-RIP1/t-RIP1, p-RIP3/t-RIP3, p-MLKL/t-MLKL, DRP1 and PGAM5. l Concentration of HMGB1. m–n Representative bands. Values are means ± SE, n = 6. a–eDifferent letters represent a significant difference, P < 0.05. IPEC-1, intestinal porcine epithelial cell 1; t-RIP1, total receptor interacting protein 1; p-RIP1, phosphorylated-receptor interacting protein 1;t-RIP3, total receptor interacting protein 3; p-RIP3, phosphorylated-receptor interacting protein 3; t-MLKL, total mixed-lineage kinase domain-like protein; p-MLKL, phosphorylated-mixed-lineage kinase domain-like protein; PGAM5, phosphoglycerate mutase 5; DRP1, dynamin-related protein 1; HMGB1, high mobility group box 1