Fig. 5
Effects of PCA and Que on tight junction protein distribution after infection with ETEC K88 in IPEC-1 cells. Cells were cultured in confocal dish with or without 40 μmol/L PCA or 10 μmol/L Que for 24 h, and then infected with or without 1 × 108 ETEC K88/mL for 2 h. a–c Tight junction protein distribution. The spread and distribution of tight junction proteins were observed through a confocal microscope. Values are means ± SE, n = 6. a–eDifferent letters represent a significant difference, P < 0.05. IPEC-1, intestinal porcine epithelial cell 1; ZO-1, zonula occludens-1