Fig. 3
Effects of PCA and Que on TEER and FD4 permeability after infection with ETEC K88 in IPEC-1 cells. Cells were cultured with or without 40 μmol/L PCA or 10 μmol/L Que for 24 h, followed by infected with or without 1 × 108 ETEC K88/mL for 1 h, 2 h and 3 h. a–c TEER. d–f FD4 permeability. Values are means ± SE, n = 6. a–cDifferent letters represent a significant difference, P < 0.05. IPEC-1, intestinal porcine epithelial cell 1; TEER, transepithelial electrical resistance; FD4, fluorescein isothiocyanate (FITC)-labeled dextran 4 kDa