Table 2 Primer and probe sequences of target and housekeeping genes
Genes | Accession numbers | Primer/probe | Sequence (5′ to 3′) |
|---|---|---|---|
β-actin | AY550069 | Forward | ACCCAGATCATGTTCGAGACC |
Reverse | TCACCGGAGTCCATCACGAT | ||
Probe | CTGTATGCCTCTGGCCGCACCA | ||
GAPDH | AF017079 | Forward | GTCGGAGTGAACGGATTTGG |
Reverse | CAATGTCCACTTTGCCAGAGTTAA | ||
Probe | CGCCTGGTCACCAGGGCTGCT | ||
FAS | AY954688 | Forward | CGTGGGCTACAGCATGATAGG |
Reverse | GAGGAGCAGGCCGTGTCTAT | ||
Probe | CATCACCA | ||
D6D | AY512561 | Forward | GACGGCCTTCATCCTTGCT |
Reverse | ACAGAGAGATGGCCGTAATCGT | ||
Probe | CCTCTCAGGCCCAGGCTGGGTG | ||
α-LA | M80520 | Forward | ACAATGGCAGCACAGAATATGG |
Reverse | TCAGTAAGGTCATCATCCAGGAATT | ||
Probe | CTCTTCCAGATCAATAAT |