Table 3 Effect of enzymatic combination (TrepCel3:TrepCel4 at 60:40) on rumen digestion and fermentation of RS, WS and LC in in vitro microbial fermentation

7.2 ± 1.60

11.5 ± 1.25

0.021

7.2 ± 1.61

11.7 ± 1.27

0.019

7.4 ± 1.63

12.2 ± 1.32

0.016

83.8 ± 1.61

101.9 ± 0.57

0.004

81.2 ± 4.40

100.2 ± 6.51

0.010

90.7 ± 2.22

104.8 ± 6.69

0.027

7.1 ± 0.07

7.1 ± 0.06

0.219

7.1 ± 0.08

7.2 ± 0.11

0.592

7.1 ± 0.08

7.1 ± 0.07

0.462

4.2 ± 0.68

5.8 ± 0.72

 < 0.001

3.7 ± 0.64

5.6 ± 1.08

 < 0.001

5.4 ± 0.98

5.9 ± 1.25

0.360

142.0 ± 24.57

170.3 ± 14.70

0.009

141.1 ± 15.10

161.6 ± 8.54

0.003

157.0 ± 18.33

188.5 ± 27.91

0.012

2.3 ± 0.15

2.5 ± 0.18

0.020

2.4 ± 0.23

2.5 ± 0.13

0.250

3.3 ± 0.35

3.4 ± 0.28

0.496

75.4 ± 2.75

89.1 ± 2.50

0.003

70.9 ± 2.5

84.8 ± 5.23

0.015

76.4 ± 1.27

84.3 ± 0.81

0.001

47.8 ± 1.95

57.2 ± 1.80

0.004

44.9 ± 1.49

54.4 ± 3.19

0.010

47.7 ± 0.60

53.5 ± 0.64

 < 0.001

17.4 ± 0.59

19.5 ± 0.65

0.014

16.3 ± 0.61

18.4 ± 1.09

0.043

17.4 ± 0.44

18.5 ± 0.10

0.014

7.3 ± 0.19

8.2 ± 0.24

0.007

7.0 ± 0.30

8.1 ± 0.62

0.057

7.9 ± 0.28

8.2 ± 0.05

0.262

2.9 ± 0.07

4.3 ± 0.25

0.001

2.7 ± 0.12

3.9 ± 0.34

0.005

3.4 ± 0.13

4.1 ± 0.04

0.001

64.2 ± 0.30

63.4 ± 0.44

0.059

64.2 ± 0.23

63.4 ± 0.23

0.01

63.5 ± 0.21

62.4 ± 0.53

0.031

21.9 ± 0.21

23.1 ± 0.21

0.002

21.7 ± 0.06

22.9 ± 0.10

 < 0.001

22.0 ± 0.10

22.8 ± 0.21

0.003

9.2 ± 0.15

9.6 ± 0.15

0.020

9.5 ± 0.17

9.9 ± 0.10

0.026

9.7 ± 0.06

10.4 ± 0.25

0.009

4.8 ± 0.36

3.9 ± 0.06

0.011

4.6 ± 0.15

3.8 ± 0.06

0.001

4.9 ± 0.01

4.4 ± 0.15

0.006

2.8 ± 0.04

2.9 ± 0.02

0.003

2.8 ± 0.02

3.0 ± 0.01

 < 0.001

2.7 ± 0.05

2.9 ± 0.02

0.007