Fig. 6

Isorhamnetin suppressed ZEA-induced ER stress in porcine oocytes. Oocytes were treated with ZEA (5 μmol/L) for 44 h in the presence or absence of isorhamnetin (10 μmol/L). A Representative images of ER distribution patterns. (a) Normal distribution. (b) Accumulative distribution. (c) Half edge distribution. (d) Almost no ER signal distribution. B Percentage of normal distribution of ER in Control, ZEA and ZEA + ISO. C Western blot of CHOP and GRP78 protein in Control, ZEA and ZEA + ISO. D–E Ratios of CHOP and GRP78 to GAPDH expression, respectively. Various letters (P < 0.05) designate significant differences. Data are presented by average ± SEM