Fig. 5
From: m6A demethylase FTO regulate CTNNB1 to promote adipogenesis of chicken preadipocyte
FTO regulate CTNNB1 expression in m6A depend manner. A The relative mRNA expression of CTNNB1 was detected after FTO overexpression or knockdown using qPCR; B The relative protein expression of β-catenin were detected after FTO overexpression or knockdown using western blot; C The expression profile of FTO and CTNNB1 during chicken preadipocyte differentiation process; D Prediction of m6A modification sites in mature CTNNB1 mRNA using online tool SRAMP; E Amplification curve and qPCR CT value in CTNNB1 420 m6A site and 414 A site after FTO overexpression; F Amplification curve and qPCR CT value in CTNNB1 2816 m6A site and 2810 A site after FTO overexpression; G RIP assays were performed to verify the interaction with FTO protein and CTNNB1 mRNA; H MeRIP assays were applied to assess that the m6A methylation level changes after FTO overexpression in chicken preadipocytes; I The RNA stability of CTNNB1 mRNAs was detected by qPCR with FTO-overexpressing in chicken preadipocytes after treatment of Act D. These values are shown as mean ± SEM of at least three biological replicates. The statistical significance of the differences was assessed using the unpaired Student's t-test (* P < 0.05; ***P < 0.001; ****P < 0.0001)