Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentae

Table 7 Rates of the net transport of water and amino acids by placentae from gilts fed diets supplemented with 0 (control) or 0.4% L-arginine (Arg) between d 14 and d 30 of gestation

Time, min	Net transport of water, nL/mg tissue/min		Net transport of Arg, pmol/mg tissue/min		Net transport of Gln, pmol/mg tissue/min		Net transport of Gly, pmol/mg tissue/min
	Control	0.4% Arg	Control	0.4% Arg	Control	0.4% Arg	Control	0.4% Arg
5	356 ± 14	475 ± 20^*	32.1 ± 2.2	58.8 ± 3.6^*	33.4 ± 2.5	64.1 ± 4.2^*	65.9 ± 3.3	123.8 ± 6.6^*
10	352 ± 17	473 ± 21^*	32.5 ± 2.0	57.3 ± 3.2^*	33.0 ± 2.2	62.8 ± 3.6^*	65.4 ± 3.8	125.2 ± 6.2^*
15	358 ± 15	479 ± 22^*	31.9 ± 1.7	56.1 ± 3.9^*	33.7 ± 2.4	63.2 ± 4.0^*	65.6 ± 3.6	124.4 ± 6.9^*

Values are means ± SEM, n = 10 gilts/treatment group. On d 30 of gestation, pieces of placental tissue (1 cm²) were mounted onto Ussing chambers, followed by the addition of either ³H₂O (20 µL), 0.2 mmol/L L-arginine (Arg) plus L-[U-¹⁴C]Arg, 0.5 mmol/L L-glutamine (Gln) plus L-[U-¹⁴C]Gln, or 1 mmol/L glycine (Gly) plus [U-¹⁴C]Gly to the “mucosal” side of the Ussing chambers for their net transport into the “serosal side” of the Ussing chambers
^*P < 0.01 vs. the corresponding control group

ISSN: 2049-1891