Blood neutrophil extracellular traps: a novel target for the assessment of mammary health in transition dairy cows

Table 3 Neutrophil extracellular traps-related indices in serum and milk of dairy cows with low- and high-DSCC at week – 1 or + 1 relative to calving¹

Items	– 1 Wk²		+ 1 Wk²			P-value³
Items	Low DSCC	High DSCC	Low DSCC	High DSCC	SEM	Hs	Wk	Hs × Wk
In serum
Neutrophil extracellular traps, ng/mL	26.4^b	40.3^a	25.2^b	41.6^a	1.16	< 0.01	0.98	0.27
Neutrophil elastase, U/L	110^ab	117^b	136^ab	138^a	3.7	0.63	< 0.01	0.53
Myeloperoxidase, U/L	230	237	259	275	12.8	0.71	0.11	0.83
Deoxyribonuclease I, U/L	99	94	104	96	2.0	0.09	0.43	0.74
In milk
Neutrophil extracellular traps, ng/mL	-	-	23.3	38.0	1.55	< 0.01	-	-
Neutrophil elastase, U/L	-	-	102	108	4.9	0.56	-	-
Myeloperoxidase, U/L	-	-	362	376	16.3	0.68	-	-
Deoxyribonuclease I, U/L	-	-	85.4	78.9	2.95	0.28	-	-

¹The data are expressed as the mean ± SEM, n = 15. ^a,b,cP < 0.05
²DSCC Differential somatic cell count, – 1 Wk Week – 1 relative to calving, + 1 Wk Week + 1 relative to calving
³Hs Health status, Wk Sampling week, Hs × Wk Interaction between health status and sampling week

ISSN: 2049-1891