Fig. 2

Effects of high stocking density exposure on intestinal barrier function and inflammatory response in broilers. A The level of interleukin 1β (IL-1β) in the jejunal mucosa; B The level of interleukin-2 (IL-2) in the jejunal mucosa; C The level of interleukin-10 (IL-10) in the jejunal mucosa; D The level of tumor necrosis factor-α (TNF-α) in the jejunal mucosa; E mRNA levels of tight junction and TLR4/NF-κB signaling pathway-related genes; F Quantitative analysis of the immunohistochemical expression of p-NF-кB; G The expression of p-NF-кB in jejunal tissues was examined by immunohistochemical analysis (200×, brown granules indicate positive reaction); NC, normal stocking density fed basal diet; PC, high stocking density fed basal diets; OA, high stocking density fed basal diets supplemented with organic acids; ^a−bValues at the same index with no common superscripts differ significantly (P < 0.05)