Pretreatment with probiotics Enterococcus faecium NCIMB 11181 attenuated Salmonella Typhimurium-induced gut injury through modulating intestinal microbiome and immune responses with barrier function in broiler chickens

Table 9 Effect of dietary Enterococcus faecium NCIMB 11181 supplementation on α-diversity of cecal microbiota of broiler chickens challenged with Salmonella Typhimurium

Items	ST¹	Clean reads/tags	Effective reads	OTUs	Chao1	Goods-coverage	Shannon	Simpson
Dosage, mg/kg
0	–	141,844	130,059	5982	5894.15	0.98	6.41	0.92
200	–	157,548	139,395	7020	7289.19	0.98	6.76	0.93
0	+	139,543	125,817	6338	6173.16	0.98	6.74	0.92
200	+	159,097	146,880	6425	6524.50	0.98	6.76	0.94
SEM²		20,771	17,075	1864	429.555	0.001	0.121	0.006
Main factors
0		140,694	127,938	6160	6033.65	0.98	6.57	0.92
200		158,318	143,138	6723	6906.84	0.98	6.76	0.94
Non-challenged		149,696	134,727	6501	6591.67	0.98	6.58	0.93
Challenged		149,320	136,349	6382	6348.83	0.98	6.75	0.93
Main factors and Interaction (P-value)³
Enterococcus faecium		0.044	0.030	0.482	0.339	0.234	0.472	0.178
Challenged		0.964	0.806	0.892	0.788	0.713	0.509	0.942
Enterococcus faecium × Challenged		0.816	0.379	0.550	0.565	0.898	0.514	0.775

¹Challenged with Salmonella Typhimurium; –, without ST challenged; + , with ST challenged
²SEM, standard error of the mean
³P-value represent the main effect of the diet, the main effect of ST challenged, and the interaction between the dietary treatments and ST challenged

ISSN: 2049-1891