Fig. 8

pHSL phosphorylation levels in bovine blastocysts. Effect of in vitro culture medium with BSA or dFCS supplemented or not with OF-EVs (D1–4) followed by UF-EVs (D5–8) on pHSL phosphorylation levels in bovine blastocysts. A pHSL phosphorylation level. Expression levels were normalized to the abundance of endogenous control actin. Phosphorylation level was expressed as pHSL/HSL. Data are expressed as means ± SEM. Different letters show statistically significant differences between treatments (P < 0.05). B Representative images of membranes treated for detection of lipolysis protein, total HSL and phosphorylated p-HSL, and β-actin. MW = molecular weight marker. Numbers indicate the MW. Expected MW: pHSL and HSL = 81 kDa and β-actin = 42 kDa