Transcriptome analysis reveals the mechanism of chronic heat stress on meat quality of broilers

Table 2 Primer sequences for real-time-quantitative-PCR analysis

Gene	GenBank number	Primer sequence (5′ to 3′)	Tm, °C	Product size, bp
ACACA	NM_205505.2	AACGAGTCGGGCTACTACCT	60.03	119
ACACA	NM_205505.2	ATCAGCATCCCGTGAAGTGG	60.11	119
LPL	NM_205282.2	TTGAAGACCCGTGCTCAGAT	59.02	249
LPL	NM_205282.2	ATCTTTCTCCCACTGCAGCT	59.01	249
PPARG	NM_001397666.1	GAATGCCACAAGCGGAGAAGGAG	63.87	88
PPARG	NM_001397666.1	GCTCGCAGATCAGCAGATTCAGG	63.72	88
SLC2A14	NM_205511.2	TCCAGGCCTTCTACAACAGG	59.02	200
SLC2A14	NM_205511.2	CCCAGCAAAAGCCAAGACAT	59.03	200
PK	NM_205469.2	GAACTGCGATGAGAATGTGC	57.55	105
PK	NM_205469.2	ACCAGCAAGGAAATGAGACC	57.79	105
LDHA	NM_205284.2	ACTTGGTCCAACGCAATGTC	59.05	168
LDHA	NM_205284.2	TGCAGCCACTACCGATAACA	59.10	168
LDHB	NM_204177.3	TGCTGAGCTCTGTGAGACAA	58.96	182
LDHB	NM_204177.3	TCAGCTGAGCCACTTCATCA	59.02	182
MYH1E	NM_001397409.1	GAGAAGAGCAAGCAGAGCCAGATG	63.51	114
MYH1E	NM_001397409.1	CAACCTTGACACGAGGATAGCACAG	63.56	114
CNN2	NM_001142256.2	GAAGATCAACCGCTCGGCACTG	64.01	139
CNN2	NM_001142256.2	CTGTGTCAGGTTCCCACTCTCAAAG	63.20	139
TMOD1	NM_205027.3	GTCTGGCATCCTGGCTCTTGTTG	63.83	81
TMOD1	NM_205027.3	GGCTGGCTCTGGTTGTCGATTC	63.74	81
PRKCQ	XM_040658916.1	ATGGTGCCCTGACACTTTGT	59.81	72
PRKCQ	XM_040658916.1	TCACTCTCAACTGGTGTGCC	59.89	72
TGFB1	NM_001318456.1	TGGATCCACGAACCCAAAGG	59.96	233
TGFB1	NM_001318456.1	CAGGCACGGACCACCATATT	60.11	233
TLR4	NM_001030693.2	CATCCCAACCCAACCACAGTAGC	63.55	119
TLR4	NM_001030693.2	CCACTGAGCAGCACCAATGAGTAG	63.46	119
β-actin	NM_205518.2	ACGTCGCACTGGATTTCGAG	60.73	282
β-actin	NM_205518.2	TGTCAGCAATGCCAGGGTAC	60.32	282

ISSN: 2049-1891