Fig. 5

LGR-1 attenuated LPS + ATP-induced NLRP3 inflammasome activity. MAC-T cells were treated with LPS (500 ng/mL) + ATP (5 mmol/L) and/or LGR-1 (MOI = 100). (A) Protein levels of NLRP3 and Caspase-1 p20. (B) Protein levels of GSDMD p30. (C) IL-1β mRNA level. (D) IL-18 mRNA level. (E) Relative protein level of NLRP3. (F) Relative protein level of Caspase-1 p20. (G) Relative protein level of GSDMD p30. The data for the CONTROL group were used to normalize the data of each treatment group. Comparisons among groups were calculated using one-way ANOVA. Data are means ± SEM of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001