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Table 6 The shear force, Hydroxyproline, collagen and IGF-1 contents, and collagen metabolism-related enzyme activities in muscle

From: Dietary protein levels changed the hardness of muscle by acting on muscle fiber growth and the metabolism of collagen in sub-adult grass carp (Ctenopharyngodon idella)

Items

Dietary protein levels

Linear

Quadratic

15.91%

19.39%

22.10%

25.59%

28.53%

31.42%

F

P

F

P

Shear force, N

2.13 ± 1.00ab

3.61 ± 0.66c

3.30 ± 0.45c

2.81 ± 0.54bc

1.86 ± 0.34a

2.12 ± 0.73ab

6.68

 < 0.05

12.55

 < 0.01

Hyp, μg/mg

0.31 ± 0.02a

0.34 ± 0.04b

0.60 ± 0.02e

0.45 ± 0.01d

0.46 ± 0.02d

0.39 ± 0.02c

64.14

 < 0.01

288.66

 < 0.01

Collagen, μg/mg

2.44 ± 0.12a

2.69 ± 0.31b

4.80 ± 0.20e

3.64 ± 0.11d

3.68 ± 0.16d

3.14 ± 0.19c

64.13

 < 0.01

288.56

 < 0.01

IGF-1, ng/mL

29.73 ± 2.18a

30.31 ± 1.74a

32.95 ± 1.51ab

33.90 ± 2.76b

33.57 ± 2.73b

30.04 ± 4.05a

1.87

0.18

10.87

 < 0.01

LOX, ng/mL

12.76 ± 0.81a

13.71 ± 0.84ab

15.23 ± 1.43b

14.72 ± 1.43b

14.65 ± 1.62b

14.06 ± 1.28ab

4.11

0.05

8.76

 < 0.01

P4H, μg/L

78.57 ± 6.39a

83.05 ± 6.56ab

88.46 ± 5.55b

84.95 ± 5.31ab

83.40 ± 7.32ab

83.28 ± 1.75ab

1.15

0.29

5.55

 < 0.05

MMP2, ng/mL

88.70 ± 1.81ab

85.50 ± 3.18ab

84.22 ± 6.01a

88.12 ± 2.88ab

89.25 ± 10.21ab

92.37 ± 5.97b

2.92

0.10

3.69

0.46

  1. Values are means ± SD and n = 6 for each group. a-e Different superscripts in the same row indicate significantly difference (P < 0.05)