Fig. 3
Effect of B. licheniformis PF9 on ETEC F4-induced gene expression of inflammatory cytokines in IPEC-J2 cells. The CON group was with no treatment. The groups of ETEC, ETEC + BL, and BL were treated with 106 CFU/mL ETEC F4 alone, both ETEC F4 (106 CFU/mL) and B. licheniformis PF9 (107 CFU/mL), and 107 CFU/mL B. licheniformis PF9 alone for 3 h, respectively. These treatment groups were also used in the following Figures. Total RNA was extracted from IPEC-J2 cells and the mRNAs abundance of TNF-α (A), IL-8 (B), and IL-6 (C) were detected by qPCR. The data were presented as mean ± SD, n = 4. Different letters represent a significant difference (P < 0.05)