Table 1 Primer sequences used for quantitative real-time PCR
Gene | Primer sequence (5ʹ to 3ʹ) | NCBI reference sequences |
|---|---|---|
Beclin1 | F: ATGGAGGGGTCTAAGGCGTC. R: TCCTCTCCTGAGTTAGCCTCT | NM_001359819.1 |
Map1lc3a | F: CATGAGCGAGTTGGTCAAGA. R: TTGACTCAGAAGCCGAAGGT | NM_025735.3 |
Ulk1 | F: AAGTTCGAGTTCTCTCGCAAG. R: CGATGTTTTCGTGCTTTAGTTCC | NM_001347394.1 |
Atg14 | F: GAGGGCCTTTACGTGGCTG. R: AATAGACGAAATCACCGCTCTG | NM_172599.4 |
Lamp1 | F: CAGCACTCTTTGAGGTGAAAAAC. R: ACGATCTGAGAACCATTCGCA | NM_001317353.1 |
Lamp2 | F: TGTATTTGGCTAATGGCTCAGC. R: TATGGGCACAAGGAAGTTGTC | NM_001017959.2 |
Mfn1 | F: GGACTTTATCCGAAACCAGA. R: TGAGATTGAAGAATGGAGGC | NM_024200.4 |
Mfn2 | F: TTCTTGTGGTCGGAGGAGTG. R: CTTTGGTGGTCCAGGTCAGT | NM_001285920.1 |
Opa1 | F: CCGAGGATAGCTTGAGGGTT. R: CGTTCTTGGTTTCGTTGTGA | NM_001199177.1 |
Drp1 | F: CAGGTGGTGGGATTGGAGAC. R: CTGGCATAATTGGAATTGGTTT | NM_001025947.2 |
Ppia | F: GAGCTGTTTGCAGACAAAGTTC. R: CCCTGGCACATGAATCCTGG | NM_008907.2 |
Rpl7 | F: TCAATGGAGTAAGCCCAAAG. R: CAAGAGACCGAGCAATCAAG | NM_011291.5 |