Fig. 6

Vitrification induced meiosis progress defection was alleviated by PCB2. A Representative images of GV, GVBD and PBE. Scale bar = 100 μm. B The rate of GVBD in different groups. C The rate of PBE in different groups. D Immunofluorescent staining of matured oocytes for α-tubulin (green) and chromosome (blue). Scale bar = 50 μm. E Representative images of euploidy and aneuploidy chromosomes. Scale bar = 10 μm. F Comparison of abnormal spindle formation in different groups. G Rate of euploidy and aneuploidy. “n” represents the cell number used in this experiment. Data are presented as mean percentage (mean ± SEM) of at least three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns non significance