Fig. 5

PCB2 can alleviate oxidative stress-related cell damage. A Representative images of early apoptotic indicator Annexin-V and autophagy indicator LC3 in different groups. DNA was counterstained with DAPI (blue). Scale bar = 50 μm. B The rate of early apoptosis. C The fluorescence intensity of LC3 signals. D Expression of Beclin1, Map1lc3a, Ulk1, Atg14, Lamp1, and Lamp2 was examined by qPCR. E Relative fluorescence intensity of γH2A.X signals. F Representative images of DNA damage in different groups. Arrow indicates polar body. Scale bar = 50 μm. “n” represents the cell number used in this experiment. Data are presented as mean percentage (mean ± SEM) of at least three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns non significance