Fig. 4
PCB2 has a positive role in regulating calcium level in vitrified oocytes. A Rhod 2-AM and Fluo 4-AM co-stained with Mito-tracker and ER-tracker respectively to indicate [Ca2+]m and [Ca2+]ER. Scale bar = 50 μm. B Representative images of cytoplasmic calcium, [Ca2+]m,and [Ca2+]ER in different groups. Scale bar = 50 μm. C-E Quantification of Fluo 3-AM, Rhod 2-AM and Fluo 4-AM fluorescence intensity. “n” represents the cell number used in this experiment. Data are presented as mean percentage (mean ± SEM) of at least three independent experiments. ***P < 0.001, ns non significance