Table 2 Primer sequences used for RT-qPCR analysis (F: forward; R: reverse)
GenBank accession No. | Primer sequence  (5' to 3') | Product length, bp |
|---|---|---|
TLR4 (NM_001293316.1) | F: CCTTTTCATCTCTGCCTTCACTAC | 112 |
R: GGGACACCACGACAATAACCT | ||
MyD88 (NM_001099923.1) | F: CTCTGGCAGCGCTCAATGTG | 105 |
R: AGTTCATCTCCTCCGCCAGC | ||
NF-κB (NM_001114281.1) | F: CTGAGGCTATAACTCGCTTGGTGAC | 131 |
R: CATGTCCGCAATGGAGGAGAAGTC | ||
TNF-α (NM_214022.1) | F: GCACTGAGAGCATGATCCGAGAC | 120 |
R: CGACCAGGAGGAAGGAGAAGAGG | ||
IL-1β (NM_214055.1) | F: GCCAACGTGCAGTCTATGGAGTG | 91 |
R: GGTGGAGAGCCTTCAGCATGTG | ||
IL-6 (NM_214399.1) | F: ATAAGGGAAATGTCGAGGCTGTGC R: GGGTGGTGGCTTTGTCTGGATTC | 93 |
IL-8 (NM_213867.1) | F: TCCAAACTGGCTGTTGCCTTCTTG R: GGGGTGGAAAGGTGTGGAATGC | 132 |
ZO-1 (XM_021098856.1) | F: TGGCATTATTCGCCTTCATAC R: AGCCTCATTCGCATTGTTT | 171 |
Occludin (NM_001163647.2) | F: TCAGGTGCACCCTCCAGATT R: AGGAGGTGGACTTTCAAGAGG | 118 |
Claudin-4 (NM_001161637.1) | F: CAACTGCGTGGATGATGAGA R: CCAGGGGATTGTAGAAGTCG | 140 |
Claudin-1 (NM_001244539.1) | F: ATTTCAGGTCTGGCTATCTTAGTTGC R: AGGGCCTTGGTGTTGGGTAA | 214 |
JAM-A (NM_001128444.1) | F: AATCAGTGTTCCCTCCTCTGCTAC R: ACGGTTGCTCTTGGGCTCT | 136 |
MUC1 (XM_001926883.5) | F: GTGCCGCTGCCCACAACCTG R: AGCCGGGTACCCCAGACCCA | 141 |
MUC2 (XM_013989745.1) | F: GGTCATGCTGGAGCTGGACAGT R: TGCCTCCTCGGGGTCGTCAC | 181 |
β-actin (AY550069) | F: ATGCTTCTAGGCGGACTGT | 211 |
R: CCATCCAACCGACTGCT |